The Ancient Forest was the first location explored in the New World and is teeming with life. The thick lush forest is home to many species of flora and fauna with many smaller ecosystems within its boundaries.
| Anjanath | Fulgur Anjanath |
| Banbaro | Bazelgeuse |
| Deviljho | Savage Deviljho |
| Ebony Odogaron | Glavenus |
| Great Jagras | Kulu-Ya-Ku |
| Kushala Daora | Leshen |
| Ancient Leshen | Nargacuga |
| Nightshade Paolumu | Pukei-Pukei |
| Rajang | Rathian |
| Rathalos | Azure Rathalos |
| Ruiner Nergigante | Tigrex |
| Tobi-Kadachi | Blackveil Vaal Hazak |
| Velkhana | Yian Garuga |
| Scarred Yian Garuga | Zinogre |
Due to the New World being a recent discovery, camps were set up around the different locals in order to help better map out areas and exploration.
| Name | Location |
|---|---|
| Southwest Camp | Area 1 |
| Northwest Camp | Area 8 |
| Northeast Camp | Area 11 |
| Ancient Forest Camp | Area 17 |


The apex monsters of the Ancient Forest are Rathalos and Nargacuga. Many resources are available in this local. Some plants can be used as weapons such as vines that can ensnare a monster. The Ancient Forest can be comparred to the Jurassic Frontier due to its many similarities. The ancient tree was formed when it consumed the bioenergy of dead Elder Dragons. The Ancient Froest recieves a lot a rain, to which a lot accumulate atop the ancient tree, and leaves the possibility of the water breaching under the right conditions. A monster of unknown origin arrived and threatened the ecosystem of the Ancient Forest, but a strange visitor fought and defeated this monster.